![]() Check out the rest of her blog, too!)īelow are links to mental health and crisis resources at Northwestern. How to read and understand a scientific paper (A guide for non-scientists, or beginning scientists.or just a very good reminder)Ī Comprehensive Guide to Applying for the NIH K99/R00 (by my wonderful friend, Anita Devineni. Is it worth the time? (That is, should you automate? A very practical xkcd!) What to do when you are overwhelmed with work (Great advice for planning and prioritizing to combat burnout.) (Superficially academia-specific, but really truly good advice generally. Words to live by!) Grant Writing Advice (Slides from Talia in PDF format)ġ2 Guidelines for Surviving Science. Path of an Academic Scientist (How to pursue a career in academic science for high school or undergrad students looking ahead) Navigating Graduate School Admissions in Science (A post by Talia giving her advice on applying to graduate school.) Observations from the Academic Job Market (A post by Talia after her experience searching for a faculty position.) Wavesurfer electrophysiology acquisition software (MatLab): Advice Some useful older fiber photometry analysis code for MatLab: GuPPy (Guided Photometry Analysis in Python, a free and open-source fiber photometry analysis tool: Introduction to Git and GitHub by Olivia Guest: In the meantime, explore the links below!Īll sorts of interesting resources and updates: 66, 486–501 (2010).Please let us know if you have a specific request. Refinement of macromolecular structures by the maximum-likelihood method. Overview of the CCP4 suite and current developments. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. G., Kontogiannis, L., Johnson, O., Powell, H. Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector. NMR chemical shifts of common laboratory solvents as trace impurities. A general method to optimize and functionalize red-shifted rhodamine dyes. Fluorescence ratio imaging of cyclic AMP in single cells. Optically detected structural change in the N-terminal region of the voltage-sensor domain. Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. A neuron-based screening platform for optimizing genetically-encoded calcium indicators. The stimulation was controlled using Wavesurfer and timing was synchronized with fluorescence acquisition using Wavesurfer and a National Instruments PCIe-6353 board. A survey of Hammett substituent constants and resonance and field parameters. Synthesis of rhodamines from fluoresceins using Pd-catalyzed C–N cross-coupling. Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. Ultrasensitive fluorescent proteins for imaging neuronal activity. ![]() Circular permutation and receptor insertion within green fluorescent proteins. Teaching old dyes new tricks: biological probes built from fluoresceins and rhodamines. A general method to fine-tune fluorophores for live-cell and in vivo imaging. A general method to improve fluorophores for live-cell and single-molecule microscopy. Bright and photostable chemigenetic indicators for extended in vivo voltage imaging. HaloTag technology: a versatile platform for biomedical applications. Beyond fluorescent proteins: hybrid and bioluminescent indicators for imaging neural activities. Chemical tags: applications in live cell fluorescence imaging. Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands. HaloTag: a novel protein labeling technology for cell imaging and protein analysis. Covalently tethered rhodamine voltage reporters for high speed functional imaging in brain tissue. Spying on neuronal membrane potential with genetically targetable voltage indicators. Isomeric tuning yields bright and targetable red Ca 2+ indicators. Fluorogenic targeting of voltage-sensitive dyes to neurons. Liu, P., Grenier, V., Hong, W., Muller, V. Single-trial imaging of spikes and synaptic potentials in single neurons in brain slices with genetically encoded hybrid voltage sensor. Genetic targeting of a voltage-sensitive dye by enzymatic activation of phosphonooxymethyl-ammonium derivative. A genetically encoded near-infrared fluorescent calcium ion indicator. Sensitive red protein calcium indicators for imaging neural activity. High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor. ![]() A high signal-to-noise Ca 2+ probe composed of a single green fluorescent protein. High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Genetically encoded indicators of neuronal activity.
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